中科院上海微系統(tǒng)與信息技術(shù)研究所王躍林/李鐵課題組與上海應(yīng)用物理研究所樊春海課題組以快報(bào)形式報(bào)導(dǎo)了他們?cè)诔哽`敏硅納米線DNA傳感器方面的合作研究進(jìn)展。

　　研究人員在傳統(tǒng)半導(dǎo)體加工技術(shù)的基礎(chǔ)上，利用硅材料自身的工藝選擇性，基于自上而下方法發(fā)展了硅納米線加工技術(shù)，并實(shí)現(xiàn)了納米級(jí)尺寸的精確控制。硅納米線不但寬度可以達(dá)到20納米，而且其三角形截面具有更大的比表面積，有利于器件性能的提高，為批量制備高檢測(cè)靈敏度的陣列FET生物傳感器邁出了堅(jiān)實(shí)的一步。

　　通過對(duì)硅納米線進(jìn)行硅烷化修飾、表面單分子膜層自組裝以及單鏈DNA探針的固定，他們研制的DNA傳感器成功實(shí)現(xiàn)了對(duì)低至1 fM 的DNA靶標(biāo)分子的響應(yīng)，這是目前已報(bào)導(dǎo)的最靈敏的基于硅納米線FET的DNA傳感器。

這種DNA傳感器還可以實(shí)現(xiàn)對(duì)單堿基錯(cuò)配的分析和對(duì)多種病原DNA序列的同時(shí)檢測(cè)。(生物谷Bioon.com)

　　Silicon-Nanowire-Based CMOS-Compatible Field-Effect Transistor Nanosensors for Ultrasensitive Electrical Detection of Nucleic Acids

　　Anran Gao , Na Lu , Pengfei Dai , Tie Li* , Hao Pei , Xiuli Gao , Yibin Gong , Yuelin Wang , and Chunhai Fan*

　　We herein report the design of a novel semiconducting silicon nanowire field-effect transistor (SiNW-FET) biosensor array for ultrasensitive label-free and real-time detection of nucleic acids. Highly responsive SiNWs with narrow sizes and high surface-to-volume-ratios were “top-down” fabricated with a complementary metal oxide semiconductor compatible anisotropic self-stop etching technique. When SiNWs were covalently modified with DNA probes, the nanosensor showed highly sensitive concentration-dependent conductance change in response to specific target DNA sequences. This SiNW-FET nanosensor revealed ultrahigh sensitivity for rapid and reliable detection of 1 fM of target DNA and high specificity single-nucleotide polymorphism discrimination. As a proof-of-concept for multiplex detection with this small-size and mass producible sensor array, we demonstrated simultaneous selective detection of two pathogenic strain virus DNA sequences (H1N1 and H5N1) of avian influenza.

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